Scuffles broke out around John Bercow’s chair as a group of MPs took a symbolic stance against Boris Johnson shutting down Parliament.
In late night drama, protests took place alongside The Speaker’s chair shortly before he stood to give his own assessment of the suspension.
A number of politicians, including Clive Lewis and Caroline Lucas, held up signs warning that they were being silenced.
Commons staff also appeared to intervene, as people fell over near the Speaker.
Labour MP for Brent Central, Dawn Butler tweeted afterwards: “Proud to be a part of the team to try and stop the prorogation of Parliament.”
Green MP Lucas also tweeted: “Joining action with fellow opposition MPs to beseech Mr Speaker not to accede to Black Rod’s request to suspend parliament, echoing the action of members to try to prevent the Speaker proroguing at the request of Charles I.”
Mr Bercow also gave a his own damning verdict of Mr Johnson’s suspension shortly after MPs rejected his second bid to call a snap election.
He told MPs: “This is not a standard or normal prorogation. It represents an act of executive fiat”.
Dozens of MPs stayed behind in the chamber – with many chanting “shame on you” as colleagues filed out the chamber.
SNP MP Hannah Bardell tweeted: “It is quite literally kicking off in the House of Commons.”
Tory MPs followed Black Rod to the Lords but Opposition MPs stayed in their seats.
Those who stayed behind sang political songs.
Labour MPs sang the Red Flag, with the SNP singing Flower of Scotland.
Welsh MPs sang Bread and Roses.
Green MP Caroline Lucas tweeted an image from the chamber of a sign saying “silenced” sitting on the Speaker’s throne.
Tory MPs were furious with Mr Bercow, who apologised to Black Rod for his intervention.
After the ceremony, which formally suspended Parliament – Mr Bercow returned to the House of Commons where he was applauded by opposition MPs.
But Tory MPs did not return to the Commons in protest at Mr Bercows announcement of his opposition.
Liberal Democrat Leader Jo Swinson outlined opposition MPs decision to protest.
She said: “The Liberal Democrats, along with other opposition parties, voted against the Prime Minister’s election this evening. We will not allow him to use an election to sneak through a dangerous No Deal Brexit, for which he has no mandate.
“We relish the chance to take on Boris Johnson in an election, but only once Article 50 has been extended. And thanks to cross-party legislation, the Prime Minister must request an extension to Article 50 rather than crash us out without a deal on the 31st October.
“As Boris Johnson can’t seem to win a vote in Parliament, he has decided to silence us until 14th October. But we will not be silenced. The Liberal Democrats will continue to vociferously and vigorously campaign for a People’s Vote, and to stop Brexit altogether.”